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Innovation
RnA
is the inventor of RnAssays® comprising assays developed at the Utrecht University and
owns patents for the innovative covalent coupling of antigenic and receptor structures to solid
surfaces, such as those of well-defined microspheres, sensor-surfaces or
sensor-tips.
We are specialized in the development and
application of time- and cost-saving diagnostic assays for safety and quality control in the
primary and secondary food production sectors with the focus on food of animal
origin.
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Food safety implies the
control of the total production process from feed to fork. Each link
in the chain of feed and food production is responsible for the
safety of the final food product.
The use of faster, more reliable and relatively
cheap tools for the control on microbiological and non-microbial
contamination are not only important for producers, but for consumers
as well.
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RnAssays®
comprise a series of easy-to-use kits to determine the presence of agents, such as
Trichinella spp., Toxoplasma gondii, sulphonamides and Salmonella spp.
These kits should meet current and future legislation demanding (fresh) food products
free from specific contaminants.
Therefore,
our kits are made suitable for a modern routine diagnostic laboratory in terms of
high-throughput, short hands-on times and improved sensitivity.
Test
results Sal Plex™ and
ELISA
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Diagnostic
sensitivity, specificity and cut-off value of the Sal Plex™ for Salmonella
serogroup B on sera relative to results of specific bacterial culturing of
lymph nodes of 1,040 Dutch slaughter pigs from various farms (field samples and
strains). The corresponding data for a frequently applied commercially
available ELISA-test kit are summarized as well.
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Positive results may be prone for
confirmation by selective bacterial culturing. The power of
the unique serogroup-fingerprinting of the Sal Plex™ is its
ability to discriminate non-specific from specific signals,
which is not possible in an ELISA assay. If a sample scores
on all serogroups then this is a very strong indication of
an occassionally occurring non-specifc antibody response
(originating from a humoral response against a non-
Salmonella Enterobacteriaceae infection).
Consequently, the chance of confirmation actions on the
basis of false-positive results will decrease and thus
their associated costs.
The precision of the Sal Plex™ outperforms
that of ELISA assays, of which the relative standard deviation is
approximately 10%. A high relative standard deviation may
give rise to false-positive results, in particular, if the
final result is close to the decision limit of the assay.
Vice versa, a smaller CV, such as for the Sal Plex™,
may give less false positive results and thus will reduce
the costs for re-analysis of the sample and that for
confirmation analyses by e.g .
selective bacterial culture.
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Literature
reference (feel free to
download)
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Fast and sensitive indirect
detection of Salmonella
spp. in meat
drip. |
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Rapid
Salmonella serology. |
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Biosensing egg yolk antibodies
against Salmonella
spp. |
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